The Cutie Mark Crusaders are a trio of fillies who havent' got their cutie marks as of yet. They consisted of Apple Bloom, Sweetie Belle, Scootaloo and recently Babs Seed. They first appeared in "My Little Pony: Friendship is Magic"
Apple Bloom-The leader of the Crusaders who met her friends at school. She is also Applejack's little sister. In "MCF: Thunder in the Jungle", she joins the MCF, Mordecai and Rigby along with Tarzan and Jane rescue Applejack from Kitten and Killer Moth.
Sweetie Belle-Rarity's sister and the third in command of the group. She is the supportive and happy-go-lucky one of the group.
Scootaloo-Rainbow Dash's surrogate little sister and biggest fan. She is the tomboy of the group and the second in command of the group.
Babs Seed-The recent member and Apple Bloom's cousin from Manehatten. Originally a bully to her cousin and her friends until the Cutie Mark Crusaders realized that she was bullying them because she was bullied back in Manehatten. They saved her life and she joined in the group after that. She'll appear in a future mystery.
- Though their debut was in "MCF and the Beanstalk", they didn't join in with the case. Instead, Othello told them the story.
- Apple Bloom so far is the only one who has joined the MCF in a case.
- Sweetie Belle is the first CMC to be captured then rescued by the MCF.
- The Cutie Mark Crusaders will make their first full MCF debut in MCF: Adventures in Ponyville.
- Mystery Case Files the Fanfiction(debut; episode: Rise of the Young Snow Queen, Sweetie Belle only)
- MCF and the Beanstalk(debut, excluding Babs Seed)
- MCF: Thunder in the Jungle(Apple Bloom only)
- MCF and the Pony Mystery (Scootaloo only)
- MCF: Adventures in Ponyville(full debut)
- MCF and the 40 Thieves(upcoming)
- MCF and the Sister Problem (excluding Babs Seed)